EC Number   |
Substrates |
Organism  |
Products |
Reversibility |
|---|
   2.4.1.140 | 2 sucrose |
- |
Leuconostoc citreum |
? |
- |
? |
| 2.4.1.140 | more |
LC/MS product analysis |
Leuconostoc citreum |
? |
- |
? |
| 2.4.1.140 | more |
alternansucrase enzyme from Leuconostoc citreum SK24.002 is used to produce di-glycosyl-stevioside through acceptor reaction. Identification of the di-glycosyl-stevioside structure as 13-([alpha-D-glucopyranosyl-(1->3)-alpha-D-glucopyranosyl-(1->6)-beta-D glucopyranosyl-(1->2)-beta-D-glucopyranosyl]oxy)kaur-16-en-19-oic acid beta-D glucopyranosyl ester, method optimization and evaluation, overview |
Leuconostoc citreum |
? |
- |
- |
| 2.4.1.140 | more |
for the docking calculations, a model of the ASRDELTA2 glucosyl-enzyme intermediate is constructed based on the high resolution structure of the GH13 covalent intermediate (PDB ID 1S46). Maltose is the best known acceptor for ASR |
Leuconostoc citreum |
? |
- |
- |
| 2.4.1.140 | more |
product analysis, determination of alternan nanoparticle size distribution by dynamic light scattering |
Leuconostoc citreum |
? |
- |
- |
| 2.4.1.140 | stevioside + sucrose |
transglucosylation |
Leuconostoc citreum |
? |
the product is composed of mono-, di-, and triglucosylated stevioside and their isomers |
? |
| 2.4.1.140 | sucrose + alpha-D-glucan |
- |
Leuconostoc citreum |
? |
- |
? |
| 2.4.1.140 | sucrose + maltose |
maltose undergoes alpha-1,6 glucosylation alone to give the oligodextran of DP3 (OD3 panose, alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc), which can be further elongated at either the O6 or the O3 position of the nonreducing unit to give the structures OD4 (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc) and OA4 (alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc), respectively. The oligosaccharide OA4 is quick to appear at the beginning of the reaction (1 min) and accumulates at a much higher level than OD4, indicating that panose is preferentially elongated with an alpha-1,3 linkage. A very small peak of OD5 originating from OD4 only appears toward the end of the reaction (after about 30 min) and is not elongated further (no OD6 is found). As soon as the OA4 starts to accumulate, it is efficiently converts to OA5 (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc). The OA5 itself can act as an acceptor for the formation of two OA6s (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-DGlc and alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc) |
Leuconostoc citreum |
? |
- |
? |
| 2.4.1.140 | 2 sucrose |
- |
Leuconostoc citreum ABK-1 |
? |
- |
? |
| 2.4.1.140 | more |
product analysis, determination of alternan nanoparticle size distribution by dynamic light scattering |
Leuconostoc citreum ABK-1 |
? |
- |
- |
