EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.2.1.12 | 2-oxobutanoate + (3E)-4-(4-chlorophenyl)but-3-en-2-one |
- |
Serratia marcescens |
? |
- |
? |
2.2.1.12 | more |
PigD catalyzes 1,2-additions using 2-oxoacids as donor substrates and aldehydes as acceptors. In the case of ketones as acceptors PigD catalyzes the 1,4-addition. Although pyruvate is the preferred donor substrate, 2-oxobutanoate is also employed using (E)-4-(4-chlorophenyl)-but-3-en-2-one as an acceptor |
Serratia marcescens |
? |
- |
? |
2.2.1.12 | pyruvate + (2E)-1,3-diphenylprop-2-en-1-one |
the PigD-catalyzed reaction yields 3% 1,4-carboligation product, with an enantiomeric excess above 99% |
Serratia marcescens |
(3R)-1,3-diphenylpentane-1,4-dione + CO2 |
- |
? |
2.2.1.12 | pyruvate + (2E)-3-(2-hydroxyphenyl)-1-phenylprop-2-en-1-one |
the PigD-catalyzed reaction yields 39% 1,4-carboligation product, with an enantiomeric excess above 99% |
Serratia marcescens |
(3R)-3-(2-hydroxyphenyl)-1-phenylpentane-1,4-dione + CO2 |
- |
? |
2.2.1.12 | pyruvate + (2E)-3-(4-methoxyphenyl)-1-phenylprop-2-en-1-one |
- |
Serratia marcescens |
(3R)-3-(4-methoxyphenyl)-1-phenylpentane-1,4-dione + CO2 |
- |
? |
2.2.1.12 | pyruvate + (3E)-4-(4-chlorophenyl)but-3-en-2-one |
39% conversion. (3R)-3-(4-Chlorophenyl)hexane-2,5-dione is produced with 75% enantiomeric excess |
Serratia marcescens |
(3R)-3-(4-chlorophenyl)hexane-2,5-dione + CO2 |
- |
? |
2.2.1.12 | pyruvate + (3E)-4-(4-chlorophenyl)but-3-en-2-one |
the PigD-catalyzed reaction yields 13% 1,4-carboligation product, with an enantiomeric excess above 99% |
Serratia marcescens |
(3R)-3-(4-chlorophenyl)hexane-2,5-dione + CO2 |
- |
? |
2.2.1.12 | pyruvate + (3E)-4-(4-hydroxy-3-methoxyphenyl)but-3-en-2-one |
- |
Serratia marcescens |
(3R)-3-(4-hydroxy-3-methoxyphenyl)hexane-2,5-dione + CO2 |
- |
? |
2.2.1.12 | pyruvate + (3E)-4-(furan-2-yl)but-3-en-2-one |
11% conversion |
Serratia marcescens |
3-(furan-2-yl)hexane-2,5-dione + CO2 |
- |
? |
2.2.1.12 | pyruvate + (3E)-4-(furan-2-yl)but-3-en-2-one |
10% conversion |
Serratia marcescens |
(3R)-3-(furan-2-yl)hexane-2,5-dione + CO2 |
- |
? |