EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.72 | more |
the enzyme methylates adenine residues in 5'-GANTC-3' sequences |
Caulobacter vibrioides |
? |
- |
- |
2.1.1.72 | more |
the DNA substrate preference for M.EcoVIII is: hemimethylated AAGCTT > AAGCTT > GAGCTT >/= CAGCTT > AATCTT > ATGCTT > ACGCTT > TAGCTT > AACCTT = single stranded AAGCTT > AGGCTT = AAACTT |
Escherichia coli |
? |
- |
- |
2.1.1.72 | more |
the enzyme complex shows strong activity on single-stranded oligo 6 T (containing GGACT), much reduced activities on 4 T (containing GGAGT) and 4B (AGACT), and no activity on oligos having two substitutions within the recognition, e.g. 6B (AGAGT) or 5 T and 5B (GGATC). Furthermore, the enzyme complex has no activity on an oligo-containing G and C only and no activity on A-containing oligos without matching consensus sequence. The enzyme complex acts on single-stranded DNA (6 T) faster than that on the corresponding single-stranded RNA and is inactive on double-stranded DNA or RNA/DNA hybrid |
Homo sapiens |
? |
- |
- |
2.1.1.72 | more |
the enzyme does not specifically bind to DNA containing GANTC sequences |
Caulobacter vibrioides |
? |
- |
- |
2.1.1.72 | more |
the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') |
Escherichia coli |
? |
- |
- |
2.1.1.72 | more |
the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine |
Haemophilus influenzae |
? |
- |
- |
2.1.1.72 | more |
the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine |
Lactococcus cremoris |
? |
- |
- |
2.1.1.72 | more |
the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine |
Haemophilus influenzae RD |
? |
- |
- |
2.1.1.72 | more |
the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine |
Lactococcus cremoris W15 |
? |
- |
- |
2.1.1.72 | more |
the DNA substrate preference for M.EcoVIII is: hemimethylated AAGCTT > AAGCTT > GAGCTT >/= CAGCTT > AATCTT > ATGCTT > ACGCTT > TAGCTT > AACCTT = single stranded AAGCTT > AGGCTT = AAACTT |
Escherichia coli E1585-68 |
? |
- |
- |