EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.186 | S-adenosyl-L-methionine + cytidine2498 in 23S rRNA |
- |
Escherichia coli |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA |
- |
? |
2.1.1.186 | S-adenosyl-L-methionine + cytidine2498 in 23S rRNA |
YgdE is the 2'-O-methyltransferase responsible for modifying 23S rRNA nucleotide C2498. Modification occurs before assembly of the 50S ribosomal subunit |
Escherichia coli |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA |
- |
? |
2.1.1.186 | S-adenosyl-L-methionine + cytidine2498 in 23S rRNA |
the recombinant YgdE methyltransferase modifies C2498 in naked 23S rRNA, but not in assembled 50S subunits or ribosomes. Nucleotide C2498 is situated within a highly conserved and heavily modified rRNA sequence, and the activity of YgdE is influenced by other modification enzymes that target this region |
Escherichia coli |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA |
- |
? |
2.1.1.186 | S-adenosyl-L-methionine + cytidine2498 in 23S rRNA |
RlmM is active on 23S rRNA from an RlmM knockout strain but not on mature 50S subunits from the same strain |
Escherichia coli |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA |
- |
? |
2.1.1.186 | S-adenosyl-L-methionine + cytidine2498 in 23S rRNA |
23S rRNA substrate structure, overview. The S-adenosyl-L-methionine-binding site is open and shallow, suggesting that RNA substrate binding may be required to form a conformation needed for catalysis. RlmM uses one side of the two domains and the inter-domain linker to recognize its RNA substrate |
Escherichia coli |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA |
- |
? |
2.1.1.186 | S-adenosyl-L-methionine + cytidine2498 in 23S rRNA |
RlmM is active on 23S rRNA from an RlmM knockout strain but not on mature 50S subunits from the same strain |
Escherichia coli BW25113 |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA |
- |
? |
2.1.1.186 | S-adenosyl-L-methionine + cytidine2498 in 23S rRNA |
23S rRNA substrate structure, overview. The S-adenosyl-L-methionine-binding site is open and shallow, suggesting that RNA substrate binding may be required to form a conformation needed for catalysis. RlmM uses one side of the two domains and the inter-domain linker to recognize its RNA substrate |
Escherichia coli BW25113 |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA |
- |
? |