EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.178 | S-adenosyl-L-methionine + cytosine1407 in 16S rRNA |
- |
Escherichia coli |
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA |
- |
? |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1407 in 16S rRNA |
nucleotide C1407 is located at a functionally active region of the 30 S subunit interface close to the P site. YebU-directed methylation of this nucleotide seems to be conserved in bacteria |
Escherichia coli |
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA |
- |
? |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1407 in 16S rRNA |
purified recombinant YebU protein retains its specificity for C1407 in vitro, and methylates 30 S subunits (but not naked 16 S rRNA or 70 S ribosomes) isolated from yebU knockout strains |
Escherichia coli |
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA |
- |
? |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1407 in 16S rRNA |
nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA |
Escherichia coli |
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA |
- |
? |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1407 in 16S rRNA |
- |
Escherichia coli BW25113 |
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA |
- |
? |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1407 in 16S rRNA |
nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA |
Escherichia coli BW25113 |
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA |
- |
? |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1407 in 16S rRNA |
- |
Escherichia coli MM294 |
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA |
- |
? |
2.1.1.178 | more |
substrate recognition mechanism, overview |
Thermus thermophilus |
? |
- |
? |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1400 in 16S rRNA |
- |
Thermus thermophilus |
S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA |
- |
? |
2.1.1.178 | S-adenosyl-L-methionine + cytosine1400 in 16S rRNA |
RsmF completely methylates C1400 when either 16S rRNA or 30S subunits are used as a substrate |
Thermus thermophilus |
S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA |
- |
? |