EC Number   |
Substrates |
Organism  |
Products |
Reversibility |
|---|
  1.8.4.11 | dabsyl-L-methionine-(S)-S-oxide + DTT |
stereospecific reduction |
Aggregatibacter actinomycetemcomitans |
dabsyl-L-methionine + DTT disulfide + H2O |
- |
? |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin |
MsrA is specific for the S-form |
Aggregatibacter actinomycetemcomitans |
L-methionine + thioredoxin disulfide + H2O |
- |
? |
| 1.8.4.11 | more |
enzyme acts on free and protein-bound methionine |
Aggregatibacter actinomycetemcomitans |
? |
- |
? |
| 1.8.4.11 | more |
role of the MsrA/MsrB repair pathway in cellular protein dynamics, mutation of gene msrA has no effect on virulence, and on resistance to oxidative agents, and causes no defect in cell envelope, msrA is probably linked to biofilm formation, enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide |
Aggregatibacter actinomycetemcomitans |
? |
- |
? |
| 1.8.4.11 | more |
the enzyme is not a major virulence determinant in the oral pathogen, MsrA is required for protein repair and protection against oxidative damage as well as for the proper expression or maintenance of functional adhesins |
Aggregatibacter actinomycetemcomitans |
? |
- |
? |
| 1.8.4.11 | peptide-L-methionine-(S)-S-oxide + thioredoxin |
stereospecific reduction |
Aggregatibacter actinomycetemcomitans |
peptide-L-methionine + thioredoxin disulfide + H2O |
- |
? |
| 1.8.4.11 | L-methionine (S)-sulfoxide + 2 dithiothreitol |
- |
Alkaliphilus oremlandii |
L-methionine + dithiothreitol disulfide + H2O |
- |
? |
| 1.8.4.11 | L-methionine (S)-sulfoxide + glutaredoxin 2 |
- |
Alkaliphilus oremlandii |
L-methionine + glutaredoxin 2 disulfide + H2O |
- |
? |
| 1.8.4.11 | more |
dunring the regeneration mechanism of MsrA by glutaredoxin, the catalytic Cys16 attacks the sulfoxide moiety of the substrate to form a sulfenic acid intermediate with the concomitant release of the product methionine. The catalytic Cys16 sulfenic acid is then attacked by glutaredoxin, leading to the formation of a complex through a mixed disulfide bond, which is reduced by glutathione, leading to MsrA regeneration |
Alkaliphilus oremlandii |
? |
- |
? |
| 1.8.4.11 | L-methionine (S)-sulfoxide + 2 dithiothreitol |
- |
Alkaliphilus oremlandii OhILAs |
L-methionine + dithiothreitol disulfide + H2O |
- |
? |
