EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.14.B11 | (R)-nicotine + O2 + [reduced NADPH-hemoprotein reductase] + O2 |
isoforms CYP82E4, CYP82E5v2, and CYP82E10 demethylate (R)-nicotine 3, 10, and 10fold faster than (S)-nicotine, respectively |
Nicotiana tabacum |
(R)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.B11 | (S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2 |
- |
Nicotiana alata |
(S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.B11 | (S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2 |
- |
Nicotiana langsdorffii |
(S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.B11 | (S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2 |
CYP82E4 selectively demethylates (S)-nicotine |
Nicotiana tabacum |
(S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.B11 | (S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2 |
only isoform CYP82E4 can significantly convert (S)-nicotine to (S)-nornicotine |
Nicotiana tabacum |
(S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.B11 | more |
isoforms CYP2A6 and CYP2B6 significantly contribute to the nicotine N-demethylation at low and high substrate concentrations, respectively |
Homo sapiens |
? |
- |
? |
1.14.14.B11 | nicotine + NADPH + O2 + H+ |
- |
Nicotiana tabacum |
nornicotine + formaldehyde + NADP+ + H2O |
- |
? |
1.14.14.B11 | nicotine + NADPH + O2 + H+ |
demethylates nicotine to form nornicotine, a precursor to the nitrosamine N'-nitrosonornicotine |
Nicotiana tabacum |
nornicotine + formaldehyde + NADP+ + H2O |
- |
? |
1.14.14.B11 | nicotine + NADPH + O2 + H+ |
six putative substrate recognition sites |
Nicotiana tabacum |
nornicotine + formaldehyde + NADP+ + H2O |
- |
? |
1.14.14.B11 | nicotine + O2 + [reduced NADPH-hemoprotein reductase] + O2 |
- |
Nicotiana tabacum |
nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |