EC Number   |
Substrates |
Organism  |
Products |
Reversibility |
|---|
   1.14.13.208 | 2-fluorobenzoyl-CoA + NADPH + H+ + O2 |
83% of the activity with benzoyl-CoA |
Aromatoleum evansii |
? |
- |
? |
| 1.14.13.208 | 4-fluorobenzoyl-CoA + NADPH + H+ + O2 |
30% of the activity with benzoyl-CoA |
Aromatoleum evansii |
? |
- |
? |
| 1.14.13.208 | benzoyl-CoA + NADPH + H+ + O2 |
the enzyme is involved in aerobic benzoate metabolism in Azoarcus evansii |
Aromatoleum evansii |
2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+ |
- |
? |
| 1.14.13.208 | benzoyl-CoA + NADPH + H+ + O2 |
no activity with NADH as electron donor |
Aromatoleum evansii |
2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+ |
- |
? |
| 1.14.13.208 | benzoyl-CoA + NADPH + H+ + O2 |
second step in aerobic benzoate metabolism, enzyme is induced by benzoate |
Aromatoleum evansii |
? |
- |
? |
| 1.14.13.208 | benzoyl-CoA + NADPH + H+ + O2 |
- |
Aromatoleum evansii |
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O |
- |
? |
| 1.14.13.208 | more |
BoxB functions as the oxygenase part of benzoyl-CoA oxygenase in conjunction with BoxA, the reductase component. BoxA functions as the reducing component of benzoyl-CoA oxygenase, which, upon binding of benzoyl-CoA, transfers two electrons to the ring in the course of dioxygenation |
Aromatoleum evansii |
? |
- |
? |
| 1.14.13.208 | more |
no activity with benzoate or phenylacetyl-CoA |
Aromatoleum evansii |
? |
- |
? |
| 1.14.13.208 | more |
purified reductase component BoxA catalyses, in the absence of BoxB, the electron transfer from NADPH to free FAD with a rate of 17 mmol/min*mg protein. This activity is very high compared to the benzoyl-CoA conversion rate with purified BoxAB (0.29 mmol/min*mg BoxA). The reductase component BoxA oxidizes NADPH to transfer electrons, mediated by enzyme-bound FAD, to one or two of the [4Fe-4S] centres of the protein. The flow of electrons from NADPH to the artificial electron acceptor, free FAD, is greatly (10-fold) stimulated by binding of benzoyl-CoA to BoxA |
Aromatoleum evansii |
? |
- |
? |
| 1.14.13.208 | more |
benzoyl-CoA epoxidase is a dinuclear iron enzyme that catalyzes the epoxidation reaction of the aromatic ring of benzoyl-CoA with chemo-, regio- and stereo-selectivity |
Aromatoleum evansii |
? |
- |
? |
