EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   5.4.99.64 | 2-hydroxy-2-methylpropanoyl-CoA |
the enzym plays a central role in the degradation of assorted substrates containing a tert-butyl moiety |
Aquincola tertiaricarbonis L108 |
(S)-3-hydroxybutanoyl-CoA |
- |
r |
| 5.4.99.64 | more |
the enzyme does not show detectable conversion of methylmalonyl-CoA and succinyl-CoA |
Aquincola tertiaricarbonis |
? |
- |
? |
| 5.4.99.64 | more |
the enzyme does not show detectable conversion of methylmalonyl-CoA and succinyl-CoA |
Aquincola tertiaricarbonis L108 |
? |
- |
? |
| 5.4.99.64 | (R)-3-hydroxybutanoyl-CoA |
very low activity with wild-type enzyme. Mutations D117V or D117A in the large subunit HcmA result in significantly increased activity toward (R)-3-hydroxybutyryl-CoA |
Aquincola tertiaricarbonis |
2-hydroxy-2-methylpropanoyl-CoA |
- |
r |
| 5.4.99.64 | 2,2-dimethylpropanoyl-CoA |
wild-type enzyme as well as I90V (HcmA) and I90A (HcmA) mutant enzymes can also isomerize pivaloyl-CoA and isovaleryl-CoA, albeit at more than 10times lower rates than the favorite substrate (S)-3-hydroxybutyryl-CoA |
Aquincola tertiaricarbonis |
? |
- |
r |
| 5.4.99.64 | isovaleryl-CoA |
wild-type enzyme as well as I90V (HcmA) and I90A (HcmA) mutant enzymes can also isomerize pivalyl- and isovaleryl-CoA, albeit at more than 10times lower rates than the favorite substrate (S)-3-hydroxybutyryl-CoA |
Aquincola tertiaricarbonis |
? |
- |
? |
