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EC Number
Substrates
Commentary Substrates
Organism
Products
Commentary (Products)
Reversibility
3.5.5.5
malononitrile + H2O
3.7% activity compared to phenylacetonitrile
Luminiphilus syltensis NOR5-1B
malonic acid + NH3
-
?
3.5.5.5
mandelonitrile + 2 H2O
-
Alcaligenes faecalis
mandelic acid + NH3
-
?
3.5.5.5
mandelonitrile + 2 H2O
-
Pseudomonas fluorescens
mandelic acid + NH3
-
?
3.5.5.5
mandelonitrile + 2 H2O
-
Alcaligenes sp.
mandelic acid + NH3
-
?
3.5.5.5
mandelonitrile + 2 H2O
-
Pseudomonas fluorescens
mandelic acid + NH3
slight preference for the formation of (R)-mandelic acid
?
3.5.5.5
mandelonitrile + 2 H2O
at 8% the rate of 4-chlorobenzylcyanide hydrolysis
Alcaligenes faecalis
mandelic acid + NH3
-
?
3.5.5.5
mandelonitrile + 2 H2O
31% enantiomeric excess for R-configuration in acid formation, 19% of product is amide with 85% enantiomeric excess for S-configuration
Pseudomonas fluorescens
mandelic acid + NH3
-
?
3.5.5.5
mandelonitrile + 2 H2O
4% activity compared to phenylacetonitrile
Bradyrhizobium japonicum
mandelic acid + NH3
-
?
3.5.5.5
mandelonitrile + 2 H2O
best substrate, 164% activity compared to phenylacetonitrile
Luminiphilus syltensis
mandelic acid + NH3
-
?
3.5.5.5
mandelonitrile + 2 H2O
-
Pseudomonas fluorescens EBC191
mandelic acid + NH3
-
?
Results
261
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270
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364
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