EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   1.14.15.8 | pregnenolone + reduced adrenodoxin + O2 |
- |
Priestia megaterium |
7beta-hydroxy-pregnenolone + oxidized adrenodoxin + H2O |
- |
? |
| 1.14.15.8 | progesterone + reduced adrenodoxin + O2 |
adrenodoxin is a [2Fe-2S] ferredoxin involved in electron transfer from NADPH+ (via a reductase) to cytochrome P-450 in the adrenal gland, 15beta-hydroxy-progesterone is the main product of wild-type enzyme and all mutants. In order to gain insights into the structure and function of CYP106A2, whose crystal structure is unknown, a homology model has been created. The substrate progesterone is then docked into the active site to predict which residues might affect substrate binding. The model is substantiated by using a combination of theoretical and experimental investigations |
Priestia megaterium |
15beta-hydroxy-progesterone + oxidized adrenodoxin + H2O |
- |
? |
| 1.14.15.8 | progesterone + reduced adrenodoxin + O2 |
adrenodoxin is a [2Fe-2S] ferredoxin involved in electron transfer from NADPH+ (via a reductase) to cytochrome P-450 in the adrenal gland |
Priestia megaterium |
15beta-hydroxyprogesterone + oxidized adrenodoxin + H2O |
- |
? |
| 1.14.15.8 | progesterone + reduced adrenodoxin + O2 |
identification of monohydroxy progesterones using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry. 15beta-Hydroxy-progesterone is the main product. 6beta-Hydroxyprogesterone, 11alpha-hydroxyprogesterone, and 9alpha-hydroxyprogesterone are formed at small amounts |
Priestia megaterium |
15beta-hydroxyprogesterone + oxidized adrenodoxin + H2O |
- |
? |
| 1.14.15.8 | progesterone + reduced adrenodoxin + O2 |
- |
Priestia megaterium |
15beta-hydroxy-progesterone + 11alpha-hydroxy-progesterone + oxidized adrenodoxin + H2O |
- |
? |
| 1.14.15.8 | progesterone + reduced ferredoxin + O2 |
Bacillus megaterium ferredoxin may be replaced by adrenal ferredoxin |
Priestia megaterium |
15beta-hydroxy-progesterone + oxidized ferredoxin + H2O |
- |
? |
| 1.14.15.8 | progesterone + reduced megaredoxin + O2 |
- |
Priestia megaterium |
15beta-hydroxyprogesterone + oxidized megaredoxin + H2O |
- |
? |
| 1.14.15.8 | progesterone + reduced megaredoxin + O2 |
megaredoxin is an iron-sulfur protein of Bacillus megaterium, megaredoxin has an apparent sulfur to iron ratio of 0.98 |
Priestia megaterium |
15beta-hydroxyprogesterone + oxidized megaredoxin + H2O |
- |
? |
| 1.14.15.8 | testosterone + reduced acceptor + O2 |
the major product is identified as 15beta-hydroxytestosterone. 6beta-Hydroxytestosterone and androst-4-ene-3,17-dione are present as minor products |
Priestia megaterium |
15beta-hydroxytestosterone + oxidized acceptor + H2O |
- |
? |
| 1.14.15.8 | testosterone + reduced adrenodoxin + O2 |
- |
Priestia megaterium |
15beta-hydroxytestosterone + oxidized adrenodoxin + H2O |
- |
? |
