1.14.13.83 | precorrin-3A + NADH + H+ + O2 |
the Pseudomonas denitrificans enzyme is active in vivo but inactive in vitro (20 mM Tris-HCl buffer, pH 8.0, with 100 mM NaCl, S-adenosyl-L-methionine (SAM), 5-aminolevulinic acid (ALA) and NADPH, aerobic, dark, 4°C), the mononuclear non-heme iron is not reducible and probably rapidly inactivated outside the bacterium |
Pseudomonas denitrificans (nom. rej.) |
precorrin-3B + NAD+ + H2O |
- |
? |