EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   1.13.12.5 | more |
establishment and evaluation of an indirect autophagy flux assay based on monitoring the degradation of an autophagosome-associated fusion protein Rluc-LC3 by luminescence detection. The Rluc-LC3 assay is useful for the identification of genes, miRNAs, and small molecules that regulate autophagy flux in mammalian cells. LC3 is a subfamily in the Atg8 family of ubiquitin-like proteins and is the only protein marker described to reliably bind the autophagosomal membrane and the phagophore Method evaluation and optimization, overview |
Renilla reniformis |
? |
- |
? |
| 1.13.12.5 | more |
substrate of Renilla luciferase, coelenterazine, is a heterocyclic imidazolo-pyrazinone, which is derivatized with (4-hydroxyphenyl)methyl (R2), 4-hydroxyphenyl (R6), and phenyl-methyl (R8) moieties |
Renilla reniformis |
? |
- |
? |
| 1.13.12.5 | n-coelenterazine + O2 |
assay at pH 7.6, about 100fold reduced relative luminescence |
Gaussia princeps |
hnu + ? |
- |
? |
| 1.13.12.5 | Renilla luciferin + O2 |
- |
Renilla reniformis |
oxidized Renilla luciferin + CO2 + hv |
- |
? |
| 1.13.12.5 | Renilla luciferin + O2 |
- |
Renilla muelleri |
oxidized Renilla luciferin + CO2 + hv |
- |
? |
| 1.13.12.5 | Renilla luciferin + O2 |
- |
Renilla koellikeri |
oxidized Renilla luciferin + CO2 + hv |
- |
? |
