EC Number |
Storage Stability |
---|
3.1.30.1 | -20°C, 50% glycerol v/v, 6 months, no loss of activity of the purified enzyme |
3.1.30.1 | -20°C, purified enzyme after dialysis against deionized water, no loss of activity |
3.1.30.1 | 4°C, 0.1 M buffer pH 3.5-4.0, mercaptoethanol, 22 h, 16.9% of initial activity at pH 3.5, 59% at pH 4.0, by preincubation with Zn2+ 48% of initial activity at pH 3.5, 86% at pH 4.0 |
3.1.30.1 | 4°C, 30 mM sodium acetate buffer pH 4.6, containing 1 mM ZnSO4, 50 mM NaCl and 5% glycerol, more than 45 days, no apparent loss of activity |
3.1.30.1 | 4°C, crude and purified form, no loss of activity after several years |