EC Number   |
Storage Stability |
|---|
    2.5.1.15 | -20°C and -80°C stable for 3 months in 20 mM TrisHCl, pH 8.0 buffer containing 10% or 20% glycerol |
| 2.5.1.15 | -20°C or -70°C, 20 mM Tris/HCl, pH 8.0, 5 weeks |
| 2.5.1.15 | -20°C or -80°C, in buffer without glycerol, 30% and 50% of the enzyme activity is lost when stored for 6 months |
| 2.5.1.15 | -20°C, enzyme remains active in 50% glycerol, 1 mM MgCl2, 5 mM 2-mercaptoethanol, stable for long term-storage, dihydropteroate synthase activity of the recombinant bifunctional fusion protein encoding dihydropterin pyrophosphokinase and dihyropteroate synthase domains of the trifunctional enzyme dihydroneopterin aldolase-dihydropterin pyrophosphokinase-dihydropteroate synthase |
| 2.5.1.15 | -20°C, sucrose, indefinitely stable |
| 2.5.1.15 | -80ºC, pH 7.0, 20% glycerol, 10-30% loss of activity in 1 month |
| 2.5.1.15 | 4°C, or -20°C, without glycerol, enzyme denaturates, dihydropteroate synthase activity of the recombinant bifunctional fusion protein encoding dihydropterin pyrophosphokinase and dihyropteroate synthase domains of the trifunctional enzyme dihydroneopterin aldolase-dihydropterin pyrophosphokinase-dihydropteroate synthase |
