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EC Number	Renatured (Commentary)	Reference
5.3.99.2	complete recovery of activity after denaturation with 2 M guanidinium hydrochloride or 4 M urea	649547
5.3.99.2	thermal unfolding is completely reversible at pH 4.0. Differential scanning calorimetry data show no concentration dependency. Presence of an intermediate state I between the native state N and the unfolded state U. Transition temperatures of the N-I and I-U transitions are 48.2 and 60.3°C, respectively. In the intermediate state, the main chain retains its characteristic beta-sheet structure without side.chain interactions	692292

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Release 2023.1 (January 2023)