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EC Number	Renatured (Commentary)	Reference
3.4.21.B7	solubilization of recombinant chitin-binding domain-tagged MAPS-1 CUB1-EGF-CUB2 from Escherichia coli strain BL21 (DE3) pLysS inclusion bodies by 7 M guanidinium hydrochloride in 50 mM Tris, 50 mM DTT, pH 8.0, followed by dilution to 5.5 mg/ml protein, and refolding by diluting 20 ml of the 5 mg/mL solution into 1 l of 0.75 M Arg, 200 mM CaCl2, 3 mM glutathione, 2 mM oxidized glutathione, pH 8.0, refolding buffer, at least one week at 4°C, followed by dialysis	732518

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Release 2023.1 (January 2023)