EC Number   |
Reference |
|---|
   3.2.2.22 | coarse-grained latice simulation model of sequence-structure modifications of the ricin A chain protein fold. Calculation of unfolding-folding transition temperature and evaluation of a possible unfolding model |
683265 |
| 3.2.2.22 | in vitro reconstitution of volkensin from separately expressed A and B chain in presence of a GSH/GSSH reduced/oxidized system |
684025 |
| 3.2.2.22 | reassociation of purified recombinant RTAs mixed with RTB in presence of 0.1 M lactose and 2% v/v 2-mercaptoethanol by dialysis for 24 h against 1 l of NaCl/phosphate solution containing 0.1 M lactose, followed by further 36 h against 5 l of NaCl/phosphate solution |
664819 |
| 3.2.2.22 | recombinant B-subunit from Escherichia coli strain BL21(DE3) inclusion bodies by denaturation in 7 M guanidinium hydrochloride, renaturation by 130fld dilution in phosphate buffer, pH 7.4, and incubation for 16 h at 4°C |
664150 |
| 3.2.2.22 | recombinant CBD-intein-Gel fusion protein expressed in Escherichia coli strain Bl21(DE3) from inclusion bodies by denaturation and stepwise dialysis, 69% of the protein is refolded to the native state in presence of 0.02 mM GSSG and 0.2 mM GSH in 10 mM Tris-HCl, 0.5 M NaCl, pH 8.5, and 0.5 M L-arginine |
666818 |
| 3.2.2.22 | recombinant functional His6-tagged enzyme from Escherichia coli inclusion bodies, method, overview |
709848 |
| 3.2.2.22 | renaturation of recombinant MLIII B-subunit from 7 M guanidinium hydrochloride by dilution with phosphate buffer, pH 7.4, and incubation for 16 h at 4°C |
664168 |
| 3.2.2.22 | study on conformation of partially unfolded state through steady-state and time-resolved fluorescence spectroscopy on a single Trp-190 which is located adjacently to the active site. The transient folding/unfolding intermediate state of beta-momorcharin adopts a specific conformation at the vicinity of the active site |
683618 |
| 3.2.2.22 | the recombinant secreted ricin-B expressed in tobacco hairy root culture is degraded in the medium within 24 h with complete loss after 72 h, the recombinant ricin-B can be recovered by aqueous two-phase extraction using PEG/potassium phosphate or PEG/sodium sulfate, the latter is more efficient |
665780 |
