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Renatured (Commentary)
Reference
3.2.1.26
in the presence of carbohydrate considerably enhances the ability of guanidine HCl-denatured extracellular invertase to be renatured relative to intracellular invertase, after incubation with 2.5 M guanidine HCl for increasing periods of time. Less than 1 min is required for maximal renaturation for both extracellular and intracellular invertase
136085
3.2.1.26
inactivated extracellular invertase can be renatured completely after renaturation with guanidine. Endo-beta-N-acetylglucosaminidase H treated extracellular invertase is restored to 40% of its original activity while intracellular invertase remains completely inactive
136086
3.2.1.26
reactivation upon heating at 80°C depends on protein concentration. Irreversible denaturation is observed for carbohydrate-depleted enzyme
655070
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