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EC Number Renatured (Commentary) Reference
Show all pathways known for 3.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.1- 709900
Show all pathways known for 3.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.1incubation in 4 M urea followed by a 15fold dilution of the urea at pH 7.5 results in a 100% recovery of initial activity after 20 or 90 min 664226
Show all pathways known for 3.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.1recombinant His-tagged enzyme from inclusion bodies after expression in Escherichia coli, solubilization by 1% SDS in ddH2O from purified inclusion bodies 653756
Show all pathways known for 3.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.1refolding behavior of guanidine hydrochloride-denatured alkaline phosphatase, despite the enhanced activity recovery yield (more than 50%), both the refolding and the reactivation rate constants decrease with increasing alpha-cyclodextrin concentrations. Detergents (used in the artificial chaperone method) increase the refolding rate significantly 663776
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