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EC Number	Renatured (Commentary)	Reference
2.7.1.105	for refolding experiments, the enzyme is first exposed to 3 M GdnHCl for 5 h at 20°C, under this condition Pfk-2 is completely unfolded, the refolding curve is obtained diluting the unfolded enzyme to several guanidine hydrochloride concentrations in the 50 mM Tris, pH 7.8, 5 mM MgCl2 and 10 mM dithiotreitol, under these conditions equilibrium is achieved after 5 h of incubation	697948

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Release 2023.1 (January 2023)