EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
3.4.22.B62 | 0.59 |
- |
Z-Val-Leu-Lys-7-amido-4-methylcoumarin |
mutant enzyme W67L, in 0.1 M sodium phosphate buffer, pH 6.0, 1 mM dithiothreitol and 1 mM EDTA at 25°C |
732674 |
3.4.22.B62 | 0.9993 |
- |
Z-Phe-Arg-NHMe |
in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C |
718252 |
3.4.22.B62 | 1.017 |
- |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin |
mutant enzyme W67L, in 0.1 M sodium phosphate buffer, pH 6.0, 1 mM dithiothreitol and 1 mM EDTA at 25°C |
732674 |
3.4.22.B62 | 1.037 |
- |
Z-Val-Leu-Lys-7-amido-4-methylcoumarin |
mutant enzyme H61N/W67L, in 0.1 M sodium phosphate buffer, pH 6.0, 1 mM dithiothreitol and 1 mM EDTA at 25°C |
732674 |
3.4.22.B62 | 1.072 |
- |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin |
mutant enzyme H61N/W67L, in 0.1 M sodium phosphate buffer, pH 6.0, 1 mM dithiothreitol and 1 mM EDTA at 25°C |
732674 |
3.4.22.B62 | 1.564 |
- |
Boc-VPR-7-amido-4-methylcoumarin |
in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C |
718252 |
3.4.22.B62 | 1.748 |
- |
Boc-VLK-7-amido-4-methylcoumarin |
in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C |
718252 |
3.4.22.B62 | 3.697 |
- |
Z-VVR-7-amido-4-methylcoumarin |
in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C |
718252 |
3.4.22.B62 | 16.78 |
- |
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin |
in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C |
694073 |
3.4.22.B62 | 20.76 |
- |
benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin |
in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C |
694073 |