EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.3.1.44 | 520 |
- |
NADH |
mutant enzyme F11K, at pH 6.2 and 25°C |
724240 |
1.3.1.44 | 180 |
- |
NADH |
mutant enzyme K245A, at pH 6.2 and 25°C |
724240 |
1.3.1.44 | 18 |
- |
NADH |
mutant enzyme Y225A, at pH 6.2 and 25°C |
724240 |
1.3.1.44 | 700 |
- |
crotonyl-CoA |
mutant F295A, pH not specified in the publication, temperature not specified in the publication |
724351 |
1.3.1.44 | 3400 |
- |
trans-2-hexenoyl-CoA |
mutant F295A, pH not specified in the publication, temperature not specified in the publication |
724351 |
1.3.1.44 | 40 |
- |
crotonyl-CoA |
mutant I287A, pH not specified in the publication, temperature not specified in the publication |
724351 |
1.3.1.44 | 2000 |
- |
trans-2-dodecenoyl-CoA |
mutant I287A, pH not specified in the publication, temperature not specified in the publication |
724351 |
1.3.1.44 | 4500 |
- |
trans-2-hexenoyl-CoA |
mutant I287A, pH not specified in the publication, temperature not specified in the publication |
724351 |
1.3.1.44 | 260 |
- |
crotonyl-CoA |
mutant L276A/V277A, pH not specified in the publication, temperature not specified in the publication |
724351 |
1.3.1.44 | 3000 |
- |
trans-2-hexenoyl-CoA |
mutant L276A/V277A, pH not specified in the publication, temperature not specified in the publication |
724351 |