EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.7.1.B3 | 16 |
- |
NADPH |
recombinant mutant F46Y, pH 7.0, 22°C |
742167 |
1.7.1.B3 | 25.6 |
- |
p-nitrobenzoic acid |
pH 7.0, 25°C |
741786 |
1.7.1.B3 | 38 |
- |
2-amino-4,6-dinitrotoluene |
pH 7.0, 37°C, recombinant His6-tagged enzyme |
743709 |
1.7.1.B3 | 50 |
- |
2,4,6-trinitrotoluene |
recombinant mutant F46Y, pH 7.0, 22°C |
742167 |
1.7.1.B3 | 65.2 |
- |
5-(aziridin-1-yl)-2,4-dinitrobenzamide |
pH 7.0, 25°C |
741786 |
1.7.1.B3 | 75 |
- |
5-(aziridin-1-yl)-2,4-dinitrobenzamide |
recombinant mutant F46Y, pH 7.0, 22°C |
742167 |
1.7.1.B3 | 80 |
- |
nitrofurazone |
recombinant mutant F46Y, pH 7.0, 22°C |
742167 |
1.7.1.B3 | 84 |
- |
2,4-dinitrotoluene |
recombinant mutant F46Y, pH 7.0, 22°C |
742167 |
1.7.1.B3 | 112 |
- |
5-(aziridin-1-yl)-2,4-dinitrobenzamide |
recombinant wild-type enzyme, pH 7.0, 22°C |
742167 |
1.7.1.B3 | 142 |
- |
2,4-dinitrotoluene |
recombinant wild-type enzyme, pH 7.0, 22°C |
742167 |