EC Number   |
General Information |
Reference  |
|---|
   4.1.99.14 | physiological function |
the overwhelming majority of DNA photoproducts in UV-irradiated spores is a unique thymine dimer called spore photoproduct, SP, or 5-thymine-5,6-dihydrothymine. This lesion is repaired by the spore photoproduct lyase enzyme that directly reverts 5-thymine-5,6-dihydrothymine to two unmodified thymines. The SP lyase enzyme demonstrates an aspect of the diversity of DNA repair mechanisms in living organisms |
680685 |
| 4.1.99.14 | more |
spectral analysis of the purified recombinant enzyme, overview |
-, 715668 |
| 4.1.99.14 | evolution |
the enzyme is a member of the radical SAM superfamily |
-, 726998 |
| 4.1.99.14 | malfunction |
the enzyme C141A mutant produces thymidylyl-(3'->5')-thymidylate likely via an unprecedented thymine radical cation reduction (proton coupled electron transfer) mechanism |
-, 726998 |
| 4.1.99.14 | more |
residue C141 is solvent exposable and no other protein residue locates between the thymidylyl-(3'->5')-thymidylate radical and the C141 in the wild--type enzyme reaction pathway |
-, 726998 |
| 4.1.99.14 | physiological function |
spore photoproduct lyase repairs a covalent UV-induced thymine dimer, spore photoproduct, in germinating endospores and is responsible for endospores' strong UV resistance. The spore photoproduct is rapidly repaired by the metalloenzyme spore photoproduct lyase when spores start germinating |
-, 726998 |
| 4.1.99.14 | evolution |
the enzyme is the first member of the radical SAM superfamily (comprising more than 44000 members) to bear a catalytically operating hydrogen atom transfer chain that is essential for S-adenosyl-L-methionine regeneration after the catalytic cycle |
-, 727020 |
| 4.1.99.14 | physiological function |
spore photoproduct lyase repairs a covalent UV-induced thymine dimer, spore photoproduct, in germinating endospores and is responsible for strong UV resistance of endospores |
-, 727020 |
| 4.1.99.14 | evolution |
the enzyme is a member of the so-called radical SAM superfamily, which is defined by the characteristic CXXXCXXC motif. The three cysteine residues serve as ligands respectively for three irons in the [4Fe-4S] cluster, with the fourth iron being coordinated by the S-adenosylmethionine in a bi-dentate manner, with its amino and carboxylate moieties serving as the fourth and fifth ligands to the cluster. SPL and the DNA photolyase, EC 4.1.99.3, show amino acid sequence homolgy and might have descended from a common ancestral protein |
-, 727074 |
| 4.1.99.14 | more |
the enzyme protects at least 9 nucleotides in the spore photoproduct containing DNA strand with 5 nucleotides 3' to and 2 nucleotides 5' to the spore photoproduct damage, suggesting that the phosphates included in this region may be involved in the binding interaction with the enzyme |
-, 727074 |
