   3.2.1.135 | evolution |
analysis and verification of neopullulanases belonging to the alpha-amylase family GH13, subfamily GH13_20, but also subfamily GH13_36, an intermediary group, which is defined using the sequence of the fifth conserved sequence region (CSR) as a selection marker. Evolutionary relationships and tree, and conserved sequence regions of GH13 subfamilies, detailed overview. The GH13_20 neopullulanase from Bacillus stearothermophilus in complex with maltotetraose is a representative of the neopullulanase subfamily. One of the most clear sequence signatures of true members of the neopullulanase subfamily subfamily GH13_20 is the stretch VANE succeeding the catalytic nucleophile in the CSR II, although not every GH13_20 member has to possess this segment in its entirety. GH13_36 enzymes contain a histidine at the end of the CSR II (strand beta4) and a tryptophan (or at least an aromatic residue) two residues succeeding the catalytic proton donor in the CSR III (strand beta5) |
731584 |
