EC Number   |
General Information |
Reference |
|---|
   2.7.1.46 | evolution |
arabinokinase is a GHMP (galactose, homoserine, mevalonate, phosphomevalonate) kinase with a unique structure that is highly conserved in plants. Isozymes Ara1 and Ara2 share 71% amino acid identity |
759961 |
| 2.7.1.46 | malfunction |
exogenous L-arabinose and its cellular accumulation are lethal for the point mutation variant ara1-1. Ara1-1 displays a lethal phenotype only upon L-arabinose treatment. The amino acid exchange of ARA1-1 is part of a conserved alpha-helix. The mutation borders a SSSAA motif which is present in both kinases and plays a role in creating the binding pocket surface. The model and alignments of both proteins show that the amino acid exchange site is closely located at the entrance of the substrate binding pocket. Recombinant Nicotiana benthamiana leaves infiltrated with ARA1 and ARA1-1 constructs show first signs of necrosis after 3 days at the sites of infiltration |
759961 |
| 2.7.1.46 | malfunction |
exogenous L-arabinose and its cellular accumulation are not lethal for the ara1-2 knockout plant. Recombinant Nicotiana benthamiana leaves infiltrated with ARA2 and ARA2-1 constructs show first signs of necrosis after 3 days at the sites of infiltration |
759961 |
| 2.7.1.46 | physiological function |
role of arabinokinase in arabinose toxicity in plants |
759961 |
| 2.7.1.46 | physiological function |
the protein is required for rice pollen development |
739344 |
