EC Number   |
General Information |
Reference |
|---|
   1.14.19.35 | evolution |
three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence |
734015 |
| 1.14.19.35 | malfunction |
a fad8 mutant shows no significant changes in its fatty acid profile at a control (22°C) temperature, while at lower temperature (15°C) the mutant shows a phenotype, leaf lipid analysis, overview |
-, 734740 |
| 1.14.19.35 | malfunction |
comparison of trienoic fatty acid levels in wild-type and fad7 mutant leaves, an increase occurs in the wild-type at 15-26°C during maturation mainly due to galactolipids enriched in plastid membranes, while in mutant leaves at 26°C the levels decrease with leaf maturation, overview |
-, 734986 |
| 1.14.19.35 | malfunction |
in a genetic background that is wild type at the FAD7 locus, the fad8 mutation has no detedable effed on overall leaf fatty acid composition irrespective of the temperature at which plants are grown. However, fatty acid analyses of individual leaf lipids reveales small decreases in the levels of 18:3 in two chloroplast lipids. In fad8 plants grown at 22°C, phosphatidylglycerol contains 22.5% 18:3 compared with 33.5% in wild type Arabidopsis |
732649 |
| 1.14.19.35 | malfunction |
in contrast to avirulent petiole exudates from wild-type plants, avirulent petiole exudates from fad7, sfd1 and sfd2 mutants are unable to activate systemic acquired resistance when applied to wild-type plants. The SAR-inducing activity is reconstituted by mixing avirulent petiole exudates collected from fad7 and sfd1 with avirulent petiole exudate from the SAR-deficient dir1 mutant |
734908 |
| 1.14.19.35 | malfunction |
leaf lipid analysis of the Arabidopsis omega-3 desaturase fad7 mutant line at growth temperature of 22°C, overview. Disruption of the AtFAD7 gene in fad7i mutant results in a reduction of TAs with an 18:3 content of 29.6% and an even more reduced content of 16:3 down to 2%. Mutant fad7i maintains 74% of 18:3 production with respect to wild-type Col-0 while only 23% of 16:3 synthesis is maintained in the mutant |
-, 745809 |
| 1.14.19.35 | malfunction |
mutant fad7i maintains 74% of 18:3 production with respect to Col-0 while only 23% of 16:3 synthesis is maintained in this mutant. The fad7/fad8 double mutant also shows a considerable reduction in trienoic fatty acids, particularly in 16:3, which is undetectable. After disruption of the AtFAD7 gene, enzyme FAD8 enzymatic activity is able to maintain, at least partially (43.7%), the amount of 18:3 and to a much lesser extent that of 16:3 (23.2%) at 22°C |
-, 734740 |
| 1.14.19.35 | malfunction |
mutations at the fad7 locus of Arabidopsis thaliana cause decreased desaturation of dienoic fatty acids in chloroplast lipids in plants grown at elevated temperatures |
732020, 732649 |
| 1.14.19.35 | malfunction |
osfad8 knockout mutant plants exhibit significant alterations in fatty acid unsaturation for all four investigated plastidic lipid classes. During a 5-day acclimation period at 4°C, further changes in fatty acid unsaturation in both wild-type and mutant plants vary according to the type of lipid. The fluidity of the thylakoid membrane are altered significantly by both FAD8 mutation and cold acclimation, suggesting that factors other than FAD8 are involved in C18 fatty acid unsaturation and fluctuations in membrane fluidity. Similarly, significant changes are noted for both the mutant and wild-type samples in terms of their fatty acid compositions as well as activities related to photosystem (PS) I, PSII, and photoprotection. This includes the development of non-photochemical quenching and increased zeaxanthin accumulation. Despite the relatively small changes in fatty acid composition during cold acclimation, cold-inducible FAD8 knock-out mutants display strong differences in photoprotective activities and a further drop in membrane fluidity. The mutants are more sensitive than wild-type to short-term low-temperature stress that results in increased production of reactive oxygen species after 5 days of chilling. The levels of 18:3 are reduced by 20% in the fad8 knockout mutant compared to wild-type, while the levels of 18:2 are increased 2fold. Phenotype, overview |
746126 |
| 1.14.19.35 | malfunction |
the omega-3 fatty acids hexadecatrienoic acid (C16:3n3), hexadecatetraenoic acid (C16:4),and alpha-linolenic acid (C18:3n3) are not detected in the CC-620 strain, phenotype and fatty acid composition of the omega-3 fatty acid deficiency in strain CC-620 due to the missense mutation detected in gene CrFAD7, complementation by the wild-type enzyme, overview |
733913 |
