EC Number   |
General Information |
Reference |
|---|
    1.12.99.6 | malfunction |
heterologous expression of the two structural genes of [FeFe]-hydrogenase in Escherichia coli yields an inactive enzyme, only the two ferredoxin-like [Fe4S4] clusters are unambiguously assembled |
703727 |
| 1.12.99.6 | more |
HybO availability is an important limiting factor for native Hyd-2 synthesis |
-, 764102 |
| 1.12.99.6 | physiological function |
during lactose fermentation by Escherichia coli mainly Hyd-4 and partially Hyd-3 affect cell growth and H2 production at different pHs. Hyd-3 and Hyd-4 are responsible for H2 production at pH 7.5, 6.5 and 5.5 |
-, 764891 |
| 1.12.99.6 | physiological function |
endogenous hydrogenases Hyd-1 and Hyd-2 induce respective 26.5 % and 36.7 % increases of succinate yield, indicating that both Hyd-1 and Hyd-2 contribute to the increased pool of reductive equivalents |
-, 764092 |
| 1.12.99.6 | physiological function |
functions as a sensory hydrogenase |
-, 704744 |
| 1.12.99.6 | physiological function |
growth of a strain that lacks a functional fructose phosphotransferase system in the presence of fructose results in an approximately 10fold increase in isoform Hyd1 levels in comparison with growth under the same conditions with glucose. This increase in the amount of Hyd1 is not due to regulation at the transcriptional level. Reintroduction of a functional fructose phosphotransferase system restores growth on D-fructose and reduces Hyd1 levels to those observed after growth on D-glucose. Reducing the rate of glucose uptake by introducing a mutation in the gene encoding the cAMP receptor protein, or consumption through glycolysis, by introducing a mutation in phosphoglucose isomerase, increased Hyd1 levels during growth on glucose. Thus the ability to oxidize hydrogen by Hyd1 shows a strong correlation with the rate of carbon flow through glycolysis and provides a direct link between hydrogen, carbon and energy metabolism |
725908 |
| 1.12.99.6 | physiological function |
Hya is responsible for hydrogen recycling during fermentation |
-, 710927 |
| 1.12.99.6 | physiological function |
in symbiotic Sesbania rostrata nodules, Azorhizobium caulinodans can use either respiratory hydrogenase to recycle endogenous H2 produced by N2 fixation |
-, 713368 |
| 1.12.99.6 | physiological function |
isoform Hya is necessary for growth after exposure to oxidative stress when hydrogen or a highly limiting concentration of acetate is the electron source. The beneficial impact of Hya on growth is dependent on the presence of H2 in the atmosphere. A Hya-deficient strain is more sensitive to the presence of superoxide or hydrogen peroxide. Hya is also required to safeguard Hyb hydrogen oxidation activity after exposure to O2. Isoform Hya is more resistant to oxidative stress than Hyb. Overexpression of Hya also results in the creation of a recombinant strain better fitted for exposure to oxidative stress than wild-type |
725269 |
| 1.12.99.6 | physiological function |
protonation is a fundamental step in hydrogen evolution at the di-iron subsite of [FeFe]-hydrogenase |
703182 |
