   3.1.1.117 | a 4-O-methyl-D-glucopyranuronate ester + H2O = 4-O-methyl-D-glucuronic acid + an alcohol |
The serine and histidine residues of the catalytic triad found in all solved GE structures (fungal and bacterial) are conserved in TtCE15A: Ser281 and His427, only the proposed canonical glutamate of the catalytic triad is absent (similar to MZ0003), and this position is occupied by a serine residue, Ser304. TtCE15A is fine-tuned to utilize Glu374 as the acidic residue in the catalytic mechanism, supporting the enzyme's high turnover rate, and that the utilization of the residue in this position is distinct from CE15 members exhibiting the canonical acidic residue |
-, 761510 |
