EC Number |
Reaction |
Reference |
---|
1.7.1.7 | IMP + NH3 + NADP+ = GMP + NADPH + H+ |
catalytic reaction mechanism, overview. GMPR catalyzes two different chemical transformations at the same active site via two different cofactor conformations, analysis by subtesla high resolution field cycling NMR relaxometry |
742897 |
1.7.1.7 | IMP + NH3 + NADP+ = GMP + NADPH + H+ |
first order reaction |
394290 |
1.7.1.7 | IMP + NH3 + NADP+ = GMP + NADPH + H+ |
mechanism: ordered sequential with GMP binding first |
394287 |
1.7.1.7 | IMP + NH3 + NADP+ = GMP + NADPH + H+ |
ordered bi-bi kinetic mechanism, in which GMP binds first to the enzyme followed by NADPH binding, and NADP+ dissociates first followed by IMP release. GMP and IMP binding are thermodynamically favorable processes. Hydride transfer contributes to the rate-limiting step, and protonation and hydride transfer steps take place in the same transition state, lending support to a concerted mechanism. Product release does not contribute to the rate-limiting step of the reaction |
725943 |
1.7.1.7 | IMP + NH3 + NADP+ = GMP + NADPH + H+ |
ordered bibi kinetic mechanism, in which GMP binds first to the enzyme followed by NADPH binding, and NADP+ dissociates first followed by IMP release |
725943 |