EC Number   |
Reaction |
Reference |
|---|
   3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
mechanism, kinetic isotope effects |
650353 |
| 3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
mechanism, overview |
651550 |
| 3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
mechanism, role of residue 182 |
649774 |
| 3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
mechanism, transition state of reaction, kinetic isotope effects, overview |
649081 |
| 3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
motif H74C(X)5R81 is involved in catalysis |
666291 |
| 3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
overview on mechanism and transition state |
651283 |
| 3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
ping-pong mechanism, catalysis proceeds through a two-step mechanism involving a phosphocysteine intermediate. In the first step, a nucleophilic cysteine thiolate attacks the phosphate ester moiety of the substrate, resulting in formation of a phosphoenzyme intermediate with release of the peptidyl tyrosine. The second step occurs via attack of water on the phosphoenzyme intermediate, and yields the final products inorganic phosphate and the regenerated enzyme. The central binding site for the substrate is the P-loop, a region at the bottom of a pocket that includes the nucleophilic cysteine and backbone amide groups oriented in a horseshoe fashion, catalytic cycle, detailed overview |
709102 |
| 3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
the phosphatase domain of PTPRs catalyze a two-step phosphate monoester hydrolysis reaction through a highly conserved sequence motif (H/V)CX5R(S/T) with a nucleophilic cysteine |
749850 |
| 3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
thermodynamic parameters |
650146 |
