EC Number   |
|---|
   7.4.2.5 | - |
| 7.4.2.5 | crude membranes containing TAPL are prepared from Sf9 insect cells, furthermore a SP Sepharose Fast Low column and a Zn2+-iminodiacetate column are used |
| 7.4.2.5 | ExeA |
| 7.4.2.5 | inverted membrane vesicles are prepared |
| 7.4.2.5 | mutant and wild-type enzymes partially by membrane fractionation |
| 7.4.2.5 | NiNTA agarose column chromatography, and gel filtration |
| 7.4.2.5 | recombinant cytosolic ATP-binding domain, N-terminal His-tag |
| 7.4.2.5 | recombinant nucleotide-binding domain |
| 7.4.2.5 | recombinant protein expressed in Escherichia coli |
| 7.4.2.5 | recombinant TAPL from membranes of Drosophila melanogaster S2 cells by detergent solubilization, and ADP or ATP affinity chromatography, not by AMP affinity |
