EC Number   |
|---|
   7.2.1.3 | - |
| 7.2.1.3 | native chromaffin granule CYB561 from adrenal gland from the chromaffin granule membrane by Triton X-100 solubilzation and separation of the solubilized nCGCytb by preparative electrophoresis. Recombinant C-terminaly His6-tagged isozyme CGCytb from Spodoptera frugiperda Sf9 cells, Pichia pastoris strain GS115, or Escherichia coli by nickel affinity chromatography to homogeneity |
| 7.2.1.3 | native enzyme from microsomal membranes by anion exchange and concanavalin A affinity chromatography, recombinant His6-tagged enzyme from Pichia pastoris by anion exchange and nickel affinity chromatography and gel filtration, elution with a solution containing 1.0% w/v n-octyl beta-glucoside |
| 7.2.1.3 | partial |
| 7.2.1.3 | purified by affinity chromatography and gel filtration |
| 7.2.1.3 | recombinant C-terminally His6-tagged isozyme DCytb from Spodoptera frugiperda Sf9 cells, and untagged, apoform or fully functional isozyme DCytb from Escherichia coli, to homogeneity |
| 7.2.1.3 | recombinant C-terminaly His6-tagged enzyme from yeast YPH499 cells by nickel affinity chromatography, recombinant C-terminally His6-tagged isozyme DCytb from Escherichia coli |
| 7.2.1.3 | recombinant untagged four CYB561 isoforms from yeast YPH499 cells partially, two recombinant C-terminally His10- or Strep-II-tagged CYB561 paralogues from Escherichia coli and Pichia pastoris by affinity chromatography |
| 7.2.1.3 | using Ni-NTA chromatography |
