EC Number |
---|
7.2.1.2 | - |
7.2.1.2 | purifiaction under strictly anoxic conditions. Solubilization is achieved with n-dodecyl beta-D-maltoside, followed by anion exchange chromatography on Q-sepharose and gel filtration on Sephacryl S300 |
7.2.1.2 | recombinant His-tagged enzyme, by Ni2+-NTA affinity chromatography |
7.2.1.2 | recombinant Rnf complex and recombinant subunits RnfC, RnfB |
7.2.1.2 | recombinant wild-type and mutant His-tagged RnfD and RnfG fom Vibrio cholerae cells by nickel affinity chromatography and gel filtration |
7.2.1.2 | Rnf complex with 6 components |