EC Number |
---|
5.2.1.4 | - |
5.2.1.4 | cell extracts are prepared |
5.2.1.4 | Ni-NTA agarose column chromatography |
5.2.1.4 | Ni-NTA column chromatography and gel filtration |
5.2.1.4 | of the recombinant enzyme |
5.2.1.4 | of the recombinant wild type and mutant enzymes |
5.2.1.4 | recombinant His6-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration |