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Search Purification (Commentary)

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Results 1 - 8 of 8
EC Number Purification (Commentary) Reference
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1- 2229, 2230, 2231, 2232, 2236, 2239, 662486, 738147
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1lysate is applied to a chelating sepharose fast flow column charged with Ni2+, the N-terminal His tag is removed by thrombin cleavage 672127
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1mutant enzymes D186A, D186N, D186E 651933
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1Ni-affinity column chromatography and Superdex G75 gel filtration 714945
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1partial 2775
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1recombinant cytosolic enzyme 651988
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1recombinant His-tagged enzyme 727770
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and diaylsis 746858
Results 1 - 8 of 8