EC Number |
Reference |
---|
5.1.3.1 | - |
2229, 2230, 2231, 2232, 2236, 2239, 662486, 738147 |
5.1.3.1 | lysate is applied to a chelating sepharose fast flow column charged with Ni2+, the N-terminal His tag is removed by thrombin cleavage |
672127 |
5.1.3.1 | mutant enzymes D186A, D186N, D186E |
651933 |
5.1.3.1 | Ni-affinity column chromatography and Superdex G75 gel filtration |
714945 |
5.1.3.1 | partial |
2775 |
5.1.3.1 | recombinant cytosolic enzyme |
651988 |
5.1.3.1 | recombinant His-tagged enzyme |
727770 |
5.1.3.1 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and diaylsis |
746858 |