EC Number   |
|---|
    5.1.1.18 | both isoforms A and B |
| 5.1.1.18 | expression in Escherichia coli with N-terminal His-tag, purification protocol from inclusion bodies |
| 5.1.1.18 | native enzyme by anion exchange chromatography, recombinant enzyme from Escherichia coli by treatment with L-arginine and anion exchange chromatography |
| 5.1.1.18 | Ni-NTA agarose column chromatography |
| 5.1.1.18 | purification of isozyme I, partial purification of isozyme II |
| 5.1.1.18 | recombinant C-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain MC1061 by nickel affinity chromatography and gel filtration |
| 5.1.1.18 | recombinant enzyme |
| 5.1.1.18 | recombinant enzyme expressed in insect cells |
| 5.1.1.18 | recombinant enzyme from Escherichia coli strain MC1061 by hydrophobic interaction and anion exchange chromatography, followed by ATP affinity chromatography and dialysis |
| 5.1.1.18 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis, to near homogeneity |
