EC Number |
---|
5.1.1.1 | - |
5.1.1.1 | alanine racemase is purified from the muscle of C. grata by using Toyopearl DEAE, Toyopearl Butyl, Hydroxyapatite and Sephacryl S200 column chromatographies. |
5.1.1.1 | alr gene encoded |
5.1.1.1 | ammonium sulfate fractionation |
5.1.1.1 | ammonium sulfate precipitation, anion-exchange chromatography, hydrophobic interaction chromatography, and gel filtration |
5.1.1.1 | by Ether-Toyopearl, Phenyl-Toyopearl and DEAE-Toyopearl column chromatographic steps |
5.1.1.1 | enzyme encoded by the dadB gene |
5.1.1.1 | enzyme overproduced in Escherichia coli W3110 lacIq |
5.1.1.1 | Ni2+-NTA column chromatography and Sephacryl S-200 gel filtration |
5.1.1.1 | Ni2+-NTA resin (His-tagged overexpressed protein), Hitrap Q-column, Superdex S prep grad column |