EC Number |
---|
4.2.99.B1 | native and recombinant protein |
4.2.99.B1 | purified from recombinant protein of an Escherichia coli expression system |
4.2.99.B1 | recombinant C-terminal 98-kDA-fragment and the mutant enzyme D829N/E830Q are purified to near homogeneity |
4.2.99.B1 | recombinant C-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli by immobilized-metal affinity chromatography and ultrafiltration |
4.2.99.B1 | recombinant His-tagged full-length enzyme Rev1 from in Escherichia coli, tag removal |
4.2.99.B1 | recombinant pol lambda and the K310A mutant are overexpressed in Eschercihia coli and purified to near homogeneity |
4.2.99.B1 | recombinant protein |
4.2.99.B1 | recombinant protein, copurification with DNA polymerase, proved by analytical gel filtration |
4.2.99.B1 | recombinant protein, expressed in Escherichia coli M15 (pREP4). The protein aggregates as inclusion bodies, solubilized in 5 M guanidinium hydrochloride and 20 mM imidazole-HCl, pH 8.0 and purified |
4.2.99.B1 | recombinant wild-type and mutant proteins H34G, K35A, K35R, Y39F, K68A, K68R, K72A, K72R, K35A/K68A, K35A/K72A, K35A/K68A/K72A, K35R/K68R/K72R are expressed in Escherichia coli and purified |