EC Number |
---|
4.1.99.11 | DEAE-Sepharose, hydroxyapatite, anion exchange chromatography, enzyme severely inactivated during purification |
4.1.99.11 | hydroxyapatite, gel filtration, 95% loss of specific activity after gel filtration |
4.1.99.11 | loss of activity during purification due to high oxygen sensitivity |
4.1.99.11 | recombinant enzyme from Escherichia coli strain BL21(DE3) |
4.1.99.11 | using a Ni-NTA-agarose column |
4.1.99.11 | using a Strep-Tactin Superflow column |