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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.B7- 723283, 724905, 725102, 725230, 725340, 725967, 726061, 726065
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.B7large scale 724163
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.B7mutant proteins K120A and K120R 724820
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.B7recombinant copurification of the RadAB complex from Escherichia coli strain Rosetta2 (DE3) DELTAarnA by cobalt affinity chromatography and gel filtration 757622
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.B7recombinant enzyme from Escherichia coli strain Rosetta by affinity and anion exchange chromatography, and dialysis, protein concentration using PEG, method, overview 757584
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.B7recombinant enzyme from Escherichia coli strain Rosetta by affinity chromatography, dialysis, anion exchange cchromatography 722331
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.B7recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)[pLysS] by nickel affinity chromatography 757864
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.B7recombinant His-tagged wild-type RadA intein and engineered minimized RadA intein from Escherichia coli strain ER2566 by nickel affinity chromatography, dialysis, His-tag cleavage through yeast ubiquitin-like specific protease, and again nickel affinity chromatography to remove the tag, followed by ultrafiltration 734472
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.B7recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) using ammonium sulfate fractionation and nickel affinity chromatography, to homogeneity 734044
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.B7recombinant MBP-tagged RadA from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, removal of the MBP-tag, all constructs contain a tobacco etch virus (TEV) protease cleavage site (ENLYFQS) following the MBP 756457
Results 1 - 10 of 11 > >>