EC Number |
---|
3.5.4.5 | - |
3.5.4.5 | Ni-NTA column chromatography |
3.5.4.5 | nickel chelating affinity column chromatography |
3.5.4.5 | Q Sepharose column chromatography, Sephacryl S-200 gel filtration, and butyl-Sepharose column chromatography |
3.5.4.5 | recombinant enzyme from Escherichia coli strain BL21 by two steps of anion exchange chromatography, and gel filtration |
3.5.4.5 | recombinant enzyme partially from 3T3 NIH and 3T3-CD3-V5 cells by ammonium sulfate fractionation |
3.5.4.5 | recombinant protein |
3.5.4.5 | recombinant wild-type enzyme by affinity chromatography on a cytidine analog 6-[3,5(cytidyl) acryloylamino]hexanoic acid resin, recombinant inactive mutants by anion exchange chromatography, ultrafiltration, and gel filtration |