EC Number   |
|---|
   3.5.1.98 | - |
| 3.5.1.98 | by affinity chromatography on a 5-ml HiTrap Chelating column charged with Ni2+ and by anion exchange chromatography on a Source 30Q column |
| 3.5.1.98 | cytoplasmic lysates are prepared |
| 3.5.1.98 | enzyme copurifies as a complex with a protein related to the retinoblastoma-associated protein, Rbap46 |
| 3.5.1.98 | partially by isolation of nuclei from Arachis hygogaea leaves, recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography |
| 3.5.1.98 | proteins are expressed in Escherichia coli BL21DE3 cells and purified using nickel nitrilotriacetic acid or TALON affinity resin, a GE HiTrap Q HP and a Superdex 26-60 column |
| 3.5.1.98 | recombinant enzyme purified aerobically from Escherichia coli contains 8fold more iron than zinc before dialysis |
| 3.5.1.98 | recombinant HDAC1 from Escherichia coli strain TOP10 by anion exchange chromatography, ultrafiltration, heparin affinity chromatography, poly-L-lysine affinity chromatography, and gel filtration. The effects of chaotropic species, weak kosmotropes, amino acids, reducing or denaturing agents, detergents, alcohols, and saturated fatty acids are tested to dissociate the protein aggregates, overview |
| 3.5.1.98 | recombinant His-tagged wild-type and mutant ARB2 domains from Escherichia coli strain BL21(DE3) by nickel affinity chroatography and gel filtration |
| 3.5.1.98 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis |
