EC Number   |
|---|
   3.5.1.87 | - |
| 3.5.1.87 | a one-step purification procedure of the recombinant L-N-carbamoylase fused to the HIS6-tag is employed using immobilized cobalt affinity chromatography |
| 3.5.1.87 | ammonium sulfate, phenyl-Sepharose CL-4B, partially purified |
| 3.5.1.87 | ammonium sulfate, phenyl-Sepharose, DEAE-cellulose, phenyl-Sepharose, Sephadex G-100 |
| 3.5.1.87 | anion-exchange chromatography, hydrophobic interaction chromatography hydroxyapatite chromatography used for purification of native expressed protein |
| 3.5.1.87 | DEAE-Sephacel, ammonium sulfate, phenyl-Sepharose CL-4B, Mono Q, Superose 12 HR |
| 3.5.1.87 | native enzyme 60fold to homogeneity by heat treatment at 70°C for 2 min, anion exchange chromatography of the supernatant, hydrophobic interaction chromatography, ultrafiltration, and gel filtration |
| 3.5.1.87 | one-step purification procedure of SmLcar mutants fused to the His6 tag employing cobalt affinity chromatography |
| 3.5.1.87 | purified 58fold to homogeneity with a yield of 16.1% by three steps of column chromatography |
| 3.5.1.87 | purified to over 95% purity |
