EC Number   |
|---|
   3.5.1.81 | - |
| 3.5.1.81 | DEAE-Toyopearl column chromatography and butyl-Cellulofine column chromatography |
| 3.5.1.81 | native enzyme 77fold to homogeneity by dialysis, several steps of anion exchange chromatography and one step of hydrophobic interaction chromatography, followed by gel filtration |
| 3.5.1.81 | native enzyme to homogeneity |
| 3.5.1.81 | native enzyme to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration |
| 3.5.1.81 | partial |
| 3.5.1.81 | recombinant enzyme |
| 3.5.1.81 | recombinant His-tagged enzyme 8.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chhromatography and ultrafiltration to homogeneity |
| 3.5.1.81 | recombinant wild-type and mutant enzymes from Escherichia coli strain JM109 by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interaction chromatography, and ultrafiltration |
| 3.5.1.81 | the enzyme is partially purified |
