EC Number   |
|---|
   3.4.21.66 | - |
| 3.4.21.66 | autolytic processes may take place during purification |
| 3.4.21.66 | native enzyme 15.3fold to homogeneity from culture supernatant by dialysis, cation exchange chromatography, and again dialysis |
| 3.4.21.66 | native enzyme from Nocardia alba strain TATA-5 by ammonium sulfate fractionation and anion exchange chromatography |
| 3.4.21.66 | native extracellular enzyme from strain KP1 by ammonium sulfate fractionation and gel filtration |
| 3.4.21.66 | recombinant enzyme from culture supernatant by dialysis and hydrophobic interaction chromatography |
| 3.4.21.66 | recombinant wild-type and mutant N-terminally maltose-binding protein-tagged and C-terminally His6-tagged enzymes from Escherichia coli strain C43(DE3) to near homogeneity from crude cell lysate by heat treatment at 80°C for 3 h, and ultrafiltration |
| 3.4.21.66 | single step fine purification |
