EC Number |
---|
3.4.17.10 | - |
3.4.17.10 | ion exchange, heparin affinity and gel filtration chromatography |
3.4.17.10 | native enzyme from brain to homogeneity by 4-aminobenzoyl affinity chromatography |
3.4.17.10 | native enzyme from pituitary to homogeneity from pituitary gland by 4-aminobenzoyl-Arg affinity chromatography with elution at pH 8.0 |
3.4.17.10 | partial |
3.4.17.10 | partially by isolation of beta-islet cells |
3.4.17.10 | recombinant GST-fusion protein from Escherichia coli strain BL21(DE3) by glutathione affinity, native pro- or mature enzyme from AtT20 cells, best at pH 5.5 in presence of Ca2+ by GST-secretogranin III affinity |