EC Number   |
|---|
   3.2.1.140 | - |
| 3.2.1.140 | Ni2+-charged HiTrap chelating column chromatography, followed by superdex 200 10/300 GL column chromatography, purity determined by SDS-PAGE with Coomassie brilliant blue R250 staining |
| 3.2.1.140 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) |
| 3.2.1.140 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Star by nickel affinity chromatography, gel filtration, and ultrafiltration |
| 3.2.1.140 | recombinant His-tagged wild-type and mutant as well as His-tagged selenomethionine-labeled enzymes from Escherichia coli by nickel affinity and anion exchange chromatography and gel filtration to homogeneity |
| 3.2.1.140 | recombinant non-tagged enzyme 3.6fold from Escherichia coli by two different steps of anion exchange chromataography and hydrophobic interaction chromatography, followed by gel filtration. Recombinant His-tagged enzyme from Escherichia coli by nickel affinity and anion exchange chromatography, and gel filtration |
| 3.2.1.140 | recombinant protein, as the C-terminally His6-tagged protein |
