EC Number   |
|---|
   3.1.4.39 | - |
| 3.1.4.39 | from CHO cells: sequential metal ion affinity and gel filtration chromatography, exchanged into phosphate-buffered saline and concentrated to 1 mg/ml of monomeric autotaxin by centrifugal filtration |
| 3.1.4.39 | from fetal bovine serum. SDS-PAGE, protein detection by silver staining |
| 3.1.4.39 | gel filtration, SDSPAGE. Establish purification protocol for recombinant hATX S48 protein. Introduction of the construction of an expression system with Escherichia coli and purification methods for the active recombinant molecule of human ATX |
| 3.1.4.39 | includes a protease inhibitor at all steps to avoid isolation of the products of proteolysis. Purified to apparent homogeneity from rat brain nuclear fractions using 1-[14C]palmitoyl-glycerophosphorylcholine as a substrate |
| 3.1.4.39 | native enzyme from brain nuclear fractions by anion exchange chromatography, gel filtration, and again anion exchange chromatography |
| 3.1.4.39 | prepared from a Vaccinia viral lysate through the concanavalin A-agarose step |
| 3.1.4.39 | recombinant ATX from HEK-293 cell culture supernatant |
| 3.1.4.39 | recombinant ATX is purified using TALON resin beads |
| 3.1.4.39 | recombinant ATX-Fc fusion protein from HEK-293 cells cell medium by affinity chromatography with protein A sepharose followed by cleavage with thrombin. Detagged ATX protein is further purified to essential homogeneity by gel filtration |
